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Paradigm AS3 Bio Autosampler, Fraction Collector, MALDI Spotters
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The Paradigm HTC PAL Liquid handler is a fully automated sample processing center that is seamlessly integrated with Paradigm MDLC instrumentation. Paradigm’s custom control of the HTC PAL provides easy, intuitive operation of the system from basic sample injection, through automated multidimensional HPLC with automated pretreatment including pH adjustment, digestion, dilution, desalting or detergent removal and injection. The PAL can be configured to hold vials, wellplates, and incorporates optional fraction collection or MALDI spotting.
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Features:
• 0.1 – 500 uL injections
• 6 x 48 vials, 96, or 384 well plates
• Temp control 4 – 400C
•Full software control
• Michrom Paradigm
• Thermo Xcalibur
• ABI/Sciex Analyst
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Options:
• Fraction Collection
• Mass Directed Purification
• Automated offline 2D HPLC
• MALDI Spotter
• Online matrix addition
• Pre-spot matrix addition
• 0.1 - 2 uL spot from capillary
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Proteomics Workstation
The Paradigm MDLC workstation employs a 4-pump MDLS coupled to an AS3 to completely automate the proteomics workflow. The proteomics workstation can isolate whole proteins or peptides through application specific mulitidimensional chromatography:
Serum Proteins: Affinity / Ion Exchange / Reversed Phase
MudPIT: Reversed Phase (pH 12)/Strong Cation Exchange/Reversed Phase (pH 3)
PTM: Strong Cation Exchange / Affinity / Reversed Phase .
Proteomics workstations provide automated desalting, buffer exchange, and detergent removal prior to injection and detection. Learn more...
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1. Mixed Bed ion exchange separation of intact protein
(WCX,WAX) with 6 salt steps. (Dimensions 1 & 2)
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2. Reversed phase separation of each salt step (Dimension 3)
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3. Eight fractions for each RP separation collected into 48 well plates
4. Trypsin digest of each protein fraction
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5. 48 30-minute reversed phase separations of digested protein 5-45%B in
20 minutes at 500 nL/min on 0.1 x 150mm HALO C18 (Dimension 4)
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48 30-minute BPT protein digests runs completed in 24 hours.
MS4-NC/AS3/ADVANCE/Linear ion trap MS/’MS
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Post-translational Modifications Workstation
The Paradigm MS4 featuring four pumps, two independent mixers, and two 10-port valves is the flagship of the Paradigm line. The MS4 is ideal for automated 1D – 4D chromatography with fractionation of complex proteome samples (proteins or peptides) prior to LCMS analysis. The MS4-NC is often used for automated online or offline Mudpit. Coupling the MS4-NC with the Michrom ADVANCE CaptiveSpray source provides higher speed, sensitivity, and robust operation for unattended overnight and weekend runs without the risk of source plugging or fouling. Learn more...
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| 96 replicate injections of protein biomarkers in complex proteome samples |
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The Paradigm HTC PAL Liquid handler is a fully automated sample processing center that is seamlessly integrated with Paradigm MDLC instrumentation. Paradigm’s custom control of the HTC PAL provides easy, intuitive operation of the system from basic sample injection, through automated multidimensional HPLC with automated pretreatment including pH adjustment, digestion, dilution, desalting or detergent removal and injection. The PAL can be configured to hold vials, wellplates, and incorporates optional fraction collection or MALDI spotting.
|
|
|
|
|
Features:
• 0.1 – 500 uL injections
• 6 x 48 vials, 96, or 384 well plates
• Temp control 4 – 400C
•Full software control
• Michrom Paradigm
• Thermo Xcalibur
• ABI/Sciex Analyst
|
Options:
• Fraction Collection
• Mass Directed Purification
• Automated offline 2D HPLC
• MALDI Spotter
• Online matrix addition
• Pre-spot matrix addition
• 0.1 - 2 uL spot from capillary
|
|
Proteomics Workstation
The Paradigm MDLC workstation employs a 4-pump MDLS coupled to an AS3 to completely automate the proteomics workflow. The proteomics workstation can isolate whole proteins or peptides through application specific mulitidimensional chromatography:
Serum Proteins: Affinity / Ion Exchange / Reversed Phase
MudPIT: Reversed Phase (pH 12)/Strong Cation Exchange/Reversed Phase (pH 3)
PTM: Strong Cation Exchange / Affinity / Reversed Phase .
Proteomics workstations provide automated desalting, buffer exchange, and detergent removal prior to injection and detection. Learn more...
|
|
1. Mixed Bed ion exchange separation of intact protein
(WCX,WAX) with 6 salt steps. (Dimensions 1 & 2)
|
|
|
|
2. Reversed phase separation of each salt step (Dimension 3)
|
|
|
|
3. Eight fractions for each RP separation collected into 48 well plates
4. Trypsin digest of each protein fraction
|
|
|
|
5. 48 30-minute reversed phase separations of digested protein 5-45%B in
20 minutes at 500 nL/min on 0.1 x 150mm HALO C18 (Dimension 4)
|
|
|
|
48 30-minute BPT protein digests runs completed in 24 hours.
MS4-NC/AS3/ADVANCE/Linear ion trap MS/’MS
|
|
|
|
|
|
|
Post-translational Modifications Workstation
The Paradigm MS4 featuring four pumps, two independent mixers, and two 10-port valves is the flagship of the Paradigm line. The MS4 is ideal for automated 1D – 4D chromatography with fractionation of complex proteome samples (proteins or peptides) prior to LCMS analysis. The MS4-NC is often used for automated online or offline Mudpit. Coupling the MS4-NC with the Michrom ADVANCE CaptiveSpray source provides higher speed, sensitivity, and robust operation for unattended overnight and weekend runs without the risk of source plugging or fouling. Learn more...
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| 96 replicate injections of protein biomarkers in complex proteome samples |
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